What causes band broadening in capillary electrophoresis?
It is widely accepted that Joule heating effects yield radial temperature gradients in capillary zone electrophoresis (CZE). The resultant parabolic profile of electrophoretic velocity of analyte molecules is believed to increase the band-broadening via Taylor-Aris dispersion.
Which of the following would elute first when using capillary zone electrophoresis?
Smaller and highly charged cations will elute first, followed by neutral compounds, which are not separated and move together in the direction of the EOF. Anions, which are strongly attracted to the positive electrode, move opposite the direction of the EOF and are eluted last.
What is the difference between capillary electrophoresis and capillary zone electrophoresis?
The key difference between capillary electrophoresis and gel electrophoresis is that gel electrophoresis is performed in a vertical or horizontal plane using a polymer gel of standard pore size whereas capillary electrophoresis is performed in a capillary tube with a polymer liquid or a gel.
What is stacking in capillary electrophoresis?
Stacking is accomplished by placing the sample in a solution whose ionic strength is significantly less than that of the buffer in the capillary tube. Because the sample plug has a lower concentration of buffer ions, the effective field strength across the sample plug is larger than that in the rest of the capillary.
What are the advantages of capillary electrophoresis?
Capillary electrophoresis (CE) provides a number of advantages for analysts, including high separation efficiency, short analysis times, low waste generation, and a diverse range of applications.
What is capillary electrophoresis used for?
Capillary electrophoresis (CE) is the primary methodology used for separating and detecting short tandem repeat (STR) alleles in forensic DNA laboratories worldwide. This chapter examines the general principles and components of injection, separation, and detection of STR alleles using CE.
What is sample stacking?
Sample stacking is a general term for methods in CE which are used for on-line concentration of diluted analytes. During the stacking process, analytes present at low concentrations in a long injected sample zone are concentrated into a short zone (stack).
What are 2 main advantages of using capillary electrophoresis?
Is capillary electrophoresis expensive?
Capillary electrophoresis was recommended due to technical advantages over agarose gel electrophoresis, most importantly the ability to measure the size of PCR products with very high resolution. Capillary electrophoresis requires expensive equipment and is not as readily available as gel electrophoresis.
What can capillary electrophoresis detect?