How thermolabile substances can be sterilized?
Sterilization of Pharmaceuticals Filtration is mainly employed for thermolabile substances and involves the passing of material through bacteria-free membrane filters. To sterilize a product, it is often necessary to perform cyclic filtration to attain a complete elimination of microorganisms.
How do you sterilize sugar solutions?
Sterilize
- Sterilize by filter sterilization (there are filters for large volumes in the storage room) OR.
- by sterilizing by Autoclaving at 110C for 10 min (short program to avoid caramelization which can affect back ground fluorescence)
How do you sterilize IV solutions?
To ensure patient safety, parenteral/injectable drug products must be sterilized to destroy any potential microbial contaminants (fungi, bacteria). The most common sterilization method involves heating under pressure in the presence of water to generate steam; this method is recommended by various pharmacopeias.
Which is best to sterilize heat labile solutions?
In order to sterilize a solution that is heat labile the best way to do this is to treat it with a chemical called ethylene oxide. This compound will sterilize anything but can be used at room temperature unlike other methods. Filtration can be done by using membrane filtrate.
Which type of evaporator is suitable for thermolabile substances?
Forced circulation evaporator
Forced circulation evaporator is suitable for thermolabile substances when evaporation is conducted under reduced pressure 2. This method is used to get concentrated insulin and liver extracts. 3. Forced circulation evaporator is used to concentrate material that deposit crystalline solid on evaporation 4.
What are the precautions that you will take while autoclaving sugar solutions?
Solutions can be autoclaved for 15-20 minutes at a maximum of 121 °C. There will be some hydrolysis to glucose and fructose, depending in part on how rapidly the autoclave comes to the required temperature and pressure. Care must be taken to prevent solutions from caramelizing.
Which of the following is not used to disinfect water?
Phenyl is not used for disinfection of drinking water.
How will you sterilize heat labile instruments?
A process for sterilizing heat-labile material using steam in an autoclave. In the first phase of the process, air in the autoclave is replaced by steam. This step also preheats the autoclave. In the second phase of the process, the material is sterilized in the presence of moisture.
How are thermolabile solutions used for sterilization?
Sterilization by filtration is done mainly for thermolabile solutions. These may be sterilized by passage through sterile bacteria-retaining filters, for e.g. membrane filters (cellulose derivatives, etc.), plastic, porous ceramic, or suitable sintered glass filters, or combinations of these.
How is the sterilization process for pharmaceutical formulations?
The invention is directed to a method for sterilizing a pharmaceutical formulation comprising a suspension of a water-insoluble pharmaceutical, comprising the steps of heat-sterilizing an aqueous solution of a viscosity enhancer, to result in a first sterile pre-mix.
What kind of filters are used for sterilization?
Sterilization by filtration is done mainly for thermolabile solutions. These may be sterilized by passage through sterile bacteria-retaining filters, for e.g. membrane filters (cellulose derivatives, etc.), plastic, porous ceramic, or suitable sintered glass filters, or combinations of these. Filters containing Asbestos should not be used.
How is moist heat used to sterilize cells?
Moist heat sterilization describes sterilization techniques that utilize saturated steam.Moist heat can denature and coagulate the protein. It can cause breakage of DNA strands and loss of functional integrity of cell membrane. Methods include: • Boiling: Boiling at 100°C is done in a water bath for 30 minutes.