What is Fluoromount G?
Description. Fluoromount-G Mounting Medium is a clear liquid medium designed for use in mounting slides following immunofluorescent staining. It is water soluble and used to mount slides in which the final step of staining is aqueous. It forms a semi-permanent seal for prolonged storage of slides at 2-8°C.
How do I get rid of Fluoromount G?
Removal of Fluoromount™ Fluoromount™ is an aqueous based mounting medium and can easily be removed by soaking the slides in deionized water: Place the slide in a beaker full of deionized water on a magnetic stirrer. Leave the slide for few hours to overnight with gentle stirring for complete removal of dry Fluoromount™
Does Fluoromount G Harden?
Fluoromount-G® Anti-Fade is a non-hardening, aqueous-based compound for mounting slides used in microscopy applications in which the preservation of fluorescence intensity is critical.
How do you use Fluoromount?
Blot excess water from the glass slide without drying the tissue specimen. The tissue must be moist prior to mounting. section and gently apply a coverslip. Allow Fluoromount to dry for 30–45 minutes at room temperature.
How do you unmount slides?
Soak the slides in xylene until the coverslips are removed. Rinse off residual medium from the tissue sections in 2 changes of fresh xylene; followed by descending grades of alcohol starting from an absolute alcohol (2 changes) ending in 95% (1 change), followed by DI water.
Which is the best mounting medium?
Popular Answers (1) As with every product recommendation, it depends on your application. There are a number of antioxidants (the antifade component) in a variety of solutions commercially available. Vectashield is probably the most widely used, and is fine for most applications.
What is the best mounting medium?
There are a number of antioxidants (the antifade component) in a variety of solutions commercially available. Vectashield is probably the most widely used, and is fine for most applications.
How is fluoromount G used in staining procedures?
Fluoromount-G® is a water-soluble compound recommended for slides mounted after a staining procedure having an aqueous final step. Since Fluoromount-G® is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened.
Can a fluoromount G Slide be removed from PBS?
This mounting medium provides a semi-permanent seal for storage of slide preparations and since Fluoromount-G ® Anti-Fade is water-soluble, the coverslip may be removed by submerging the slide in a PBS solution until the coverslip is loosened.
How is fluoromount G used to treat breast cancer?
MDA-MB-231 cells were stained with conjugated phalloidin and mounted with Fluoromount-G® (SB Cat. No. 0100-01). Image from McConnell BV, Koto K, Gutierrez-Hartmann A. Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.
What is the refractive index of fluoromount G?
This mounting medium also provides a semi-permanent seal for storage of slide preparations. The refractive index of Fluoromount-G® is reported to be ~1.4 70 .