What is GM-CSF protein?
GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes.
How do you reconstitute GM-CSF?
Reconstitution Recommendation: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Lyophilized recombinant human GM-CSF should be reconstituted in sterile, distilled water to 0.1-1.0 mg/mL to regain full activity.
What is the difference between M CSF and GM-CSF?
M-CSF exhibits a mostly homeostatic expression pattern, whereas GM-CSF is a product of cells activated during inflammatory or pathologic conditions. Accordingly, M-CSF regulates the numbers of various tissue macrophage and monocyte populations without altering their “activation” status.
What cells make GM-CSF?
GM-CSF is produced by various cell types including macrophages, mast cells, T cells, fibroblasts and endothelial cells 8, 9, mostly in response to immune activation and cytokines that mediate inflammation.
Is G-CSF a cytokine?
Granulocyte colony-stimulating factor (G-CSF) is the major cytokine regulator of neutrophilic granulocytes [3]. Neutrophils are an essential cell type in the innate immune system necessary for the clearance of bacterial pathogens.
Is the recombinant murine GM CSF cross species reactivity?
The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues. Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Where is GM-CSF produced in the body?
It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.
What is the biological activity of CSF peprotech?
Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Not for human use.
What is the function of GM-CSF in neutrophils?
GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.