How big is an A549 cell?
Results: (1) The diameter of A549 cells from inverted microscopy and TEM images was 14.93 μm and 10.59 μm. (2) By defining cell as reference space the volume densities (VV) of nucleus and cytoplasm were about 0.28 and 0.72; the surface densities (SV) of nucleus were 0.19 μm-1.
How do A549 cells grow?
A549 cells are cultured in complete media consisting of Dulbecco’s MEM modified with 10% FBS. The cells can be grown as adherent or in suspension in vitro. The A549 cell line grows easily and cell count doubling time is typically 24-40 hours.
Where are A549 cells found?
A549 cells, as found in the lung tissue of their origin, are squamous and responsible for the diffusion of some substances, such as water and electrolytes, across alveoli. If A549 cells are cultured in vitro, they grow as a monolayer; adherent or attaching to the culture flask.
Why are A549 cells used?
The A549 cells have been used to model the alveolar Type II pulmonary epithelium. Studies have shown that this can be particularly useful in research for studying the metabolic processing of lung tissue and for identifying mechanisms of drug delivery to the tissue.
Where did the A549 cell line come from?
Scientists at the National Cancer Institute developed a cell line designated A549 that was derived from explanted cultures of human lung cancer tissue.
How is the A549 NSCLC cell line cultured?
The A549 non-small-cell lung carcinoma (NSCLC) cell line is cultured and then inoculated in mice to create the A549 CDX mouse model (Cell Line Derived Xenograft).
Can A549 cells be used in clinical trials?
The A549 cell line has been tested under the guidance of the United States Food and Drug Administration (FDA) so, under current Good Manufacturing Practices (GMP), these cells may be suitable for use in manufacturing constructs for use in clinical trials. The National Cancer Institute seeks parties to non-exclusively license this research material.
Is the A549 cell line suitable for adenovirus production?
The A549 cell line has also been found to be suitable for adenovirus production, most notably replicating adenovirus constructs that do not require complementation by the viral oncogene, early region 1A (E1A), which is responsible for viral gene transcription. This cell line is further utilized as a negative control in