Is concanavalin A lectin?
Concanavalin A (Con A) is a plant lectin that is purified from jack beans. Con A binds to the mannose residues of various glycoproteins and activates lymphocytes.
What is the function of Concanavalin A?
Concanavalin A (Con A), a mannose/glucose-binding lectin isolated from Jack beans (Canavalia ensiformis), is a well-known T cell mitogen that can activate the immune system, recruit lymphocytes and elicit cytokine production [1].
What does Concanavalin a bind to?
Fluorescein-labeled Concanavalin A is able to bind to the cell wall of algal cells recently isolated from Evernia prunastri and Xanthoria parietina thalli. This binding involves a ligand, probably a glycoprotein containing mannose, which can be isolated by affinity chromatography.
What sugar does Concanavalin a bind to?
The carbohydrate-binding specificity of Con A has been studied in great detail by every conceivable technique. It binds d-glucose, d-fructose, d-mannose, N-acetyl-d-glucosamine and related monosaccharides [3,8], The α-anomer of d-mannose is the monosaccharide most complementary to the Con A sugar binding site.
Is concanavalin A toxin?
In this context we propose an experimental study on Concanavalin A (Con A) aggregation and use it as a model to study the relationship between cell toxicity and aggregation processes. This happens because they are recruited in the mature fibrillar structure which-as a consequence-turns out to be non-toxic.
What is meant by concanavalin?
: a protein that occurs in the jack bean and is a mitogen and hemagglutinin.
Is concanavalin A A primary metabolite?
Complete Step by Step answer: Concanavalin A is a secondary metabolite e.g. is a lectin, it has the property to agglutinate RBCs. These are required for basic metabolic processes. These are derivatives of primary metabolites and are not required for the basic metabolic processes. These are easily extracted from plants.
Which group has only secondary metabolites?
Glycine, gums, serine, diterpenes.
Is concanavalin A secondary metabolites?
Complete Step by Step answer: Concanavalin A is a secondary metabolite e.g. is a lectin, it has the property to agglutinate RBCs. These are derivatives of primary metabolites and are not required for the basic metabolic processes. These are easily extracted from plants.
How are proteins able to bind to Concanavalin A?
The proteins containing the sugars can bind; those that lack the sugars, don’t bind. The proteins that bind to the concanavalin A can generally be eluted (detach) from the Con A by either changing the pH, the ionic strength, or by adding sugars that compete for the binding sites on the conA.
Where can I get concanavalin in the lab?
REFERENCES: Pharmacia-LKB notes on the resin; Pharmacia book on Affinity Chromatography; for the structure of mannopyranose and methyl-glucopyranoside, see Morrison & Boyd, Organic Chemistry (in the 3rd edition, see pages 1104-1105). Concanavalin A is a lectin (typically obtained from wheat germ).
How to make Willott supernatant with ConA buffer?
Add to 15 mls with conA buffer, invert the tube to mix, then centrifuge 5 min 500g and discard supernatant. Resuspend in 7 mls conA buffer and transfer the resin to a small column with fitted frit.