# Why is Hanes Woolf more accurate than Lineweaver-Burk?

## Why is Hanes Woolf more accurate than Lineweaver-Burk?

Just like the Lineweaver-Burk equation, the Hanes-Woolf equation is of the form y = mx + c. The Hanes-Woolf plot is thought to be more accurate than Lineweaver-Burk for the determination of kinetic parameters.

### Which of the following is known as Eadie-Hofstee plot?

Eadie-Hofstee Plot is also known as Woolf-Eadie-Augustinsson-Hofstee or Eadie- Augustinsson plot. This is a graphical representation of enzyme kinetics in which velocity is plotted as a function of the ratio between velocity and substrate concentration. This can be derived from the Michaelis-Menten equation.

#### What is the main disadvantage of the Eadie-Hofstee plot as compared to the Lineweaver-Burk plot?

The V-intercept in Lineweaver-Burke plot is equivalent to the inverse of Vmax due to which the experimental error gets magnified. Similarly the Eadie-Hofstee plot has the disadvantage that v appears on both axes. Experimentally, this quantity is generally subject to more error than substrate concentration.

What is the slope of the Lineweaver-Burk plot?

A Double-Reciprocal or Lineweaver-Burk Plot. A double-reciprocal plot of enzyme kinetics is generated by plotting 1/V0 as a function 1/[S]. The slope is the KM/Vmax, the intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis (more…)

How do you plot Michaelis Menten?

Plotting the Michaelis-Menten Curve Label the x-axis mM of [S] or concentration of substrate. Label the y ax- sec/micro-mole of V or velocity of reaction. Insert different values of [S] into the Michaelis-Menten equation, along with the values found for Km and Vmax, to solve for V.

## Why is Lineweaver-Burk plot not accurate?

As a double reciprocal plot, the Lineweaver-Burk plot presents two problems when used with real life experimental data. First, all data found at large substrate concentrations will be clustered near the origin. Thus relying too heavily on the points far from the origin can lead to inaccurate values of KM and Vmax.

### Why is a Lineweaver-Burk plot useful?

Uses of Lineweaver–Burk Plot Used to determine important terms in enzyme kinetics, such as Kmand Vmax, before the wide availability of powerful computers and non-linear regression software. Gives a quick, visual impression of the different forms of enzyme inhibition.

#### How is the Hanes-Woolf plot used in biochemistry?

In biochemistry, a Hanes–Woolf plot is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration [S] to the reaction velocity v is plotted against [S]. It is based on the rearrangement of the Michaelis–Menten equation shown below: where Km is the Michaelis–Menten constant…

Why was the plot rearranged in Hanes Woolf?

J B S Haldane stated, reiterating what he and K. G. Stern had written in their book, that this rearrangement was due to Barnet Woolf. However, it was just one of three transformations introduced by Woolf, who did not use it as the basis of a plot. There is therefore no strong reason for attaching his name to it.

Why did C’s Hanes not use a plot?

It was first published by C. S. Hanes, though he did not use it as plot either. Hanes said that the use of linear regression to determine kinetic parameters from this type of linear transformation is flawed, because it generates the best fit between observed and calculated values of

## Who is the author of the Hanes plot?

However, it was just one of three transformations introduced by Woolf, who did not use it as the basis of a plot. There is therefore no strong reason for attaching his name to it. It was first published by C. S. Hanes, though he did not use it as plot either.